Ts-/Cs- Mutants

Tiffany had been trying to insert the YTM1 gene into pRS315 so that I could use the plasmid in my screens for Ts- and Cs- mutants. She had also been trying to insert ytm1-1 into pSLS315 for a future synthetic lethal screen. I mini prepped the transformants that resulted from her ligation reactions using the BioRad kit. Digests of both plasmids showed that neither ligation was successful. Because we were having so much trouble with the pRS315 ligation reaction, I decided to focus on finding dominant mutants of Ytm1p.