happy ending
to my work day at least. my pcr worked the second time, although not with my plasmid. it's probably a problem with the mini prep method. i get to use the kit tomorrow, woohoo! and the digest came out good too. i found a restriction enzyme that cuts twice in the gene insert and never in the vector so i can easliy cut most of the insert out of the vector. hooray! i'd post some gels or something but i don't have them saved on this computer. sad. ah well. now i get to tutor writing (ha ha) and then go see a show!
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