blah blah blah

so i set up my site directed mutagenesis reactions. that was exciting. now they are pcring in the hot lab and i am sitting here killing time. ah.... perhaps by friday i will have plasmids with two snabi restriction sites in them. that would be wonderful. in the meantime, i'm also hoping that some transformants will grow on my plates from yesterday. that would also be wonderful. and if not, or really even if there are transformants, i get to trouble-shoot and optimize my pcr mutageniesis reactions until i get a good amount of transformants. then maybe some of them will contain dominant-negative mutations in the ytm1 protein that can kill my cells!! however, i doubt i'll reach that point by the end of the summer. i've been here for almost five weeks already and haven't accomplished much of anything. unless you count the fact that i've figured out that the plasmid i get to work with is a big suck. makes it more difficult and also more interesting i suppose.

if i can manage to kill another half hour or so then i can leave and it will be all good.

i get to tutor today. writing. i don't know who thought it would be a good idea for me to tutor writing. not that i'm a bad writer or anything, i just don't remember any of the reasons behind any grammar rules at all. so when i try to explain why you would want to put a comma or something somewhere, i can never come up with a justification for it. i'm always like, "use this word because it flows better" or something equally stupid. ugh.

the other interesting thing i did today when i was bored was to read through my past blog entries. yeah i was that bored. it was kinda neat though. i started this blog at the end of last summer when i was bored out of my mind working at scherring-plough (the pharmaceutical company that makes claritin). it was interesting to compare my posts from the summer months to my posts from the school year. during the summer i'm mostly happy but bored. this summer definitely less bored than the last. and during the school year i'm either drunk after a party or really depressed and stresser out. not cool. i should try to avoid that happening. it's just not fun.

i really think next year will be better. i have some really fun and easy classes to look forward to. and also physical biochemistry but i don't want to think about that. i'm still going to be pretty heavily involved in sns but at least i will be less politically involved. and i haven't decided if i'm going to propose to direct anything this year. i'm leaning towards no right now, but i haven't made up my mind. it could be a lot of fun, but i would also be incredibly stressed out and disappointed all over again. i don't know if i want to do that again. plus, i'd really like a chance to be on stage again. even in something small like sotr or something. i miss acting and singing and dancing an awful lot. eh, we'll see what happens i suppose.

tomorrow will be a semi busy day of work, but not really. i have a bunch of different things to do, including a digest and a pcr. and i also have to finish up my site directed mutagenesis stuff, which involves a restriction digest and a bacterial transformation using electroporation (that's electricity to poke little holes in the cells so the dna can go in, non-biodorks). but most of that stuff involves a lot of set up and a lot of incubation. so i will have a good amount of random free time.

tonight i some movie and tomorrow night is some other movie and then friday is a cake concert for free. at least my evenings are anything but boring!

and i will remember to bring my sneakers to tutoring with me so i can go running in the uc afterwards! hooray!

blueberry cornbread from whole foods is sooooo good! and i also got a big tub of crab artichoke dip. i just have to make some bread and all will be wonderful. and i'll get fatter. like the pudgy fluffy!! woohoo!